FastQCFastQC Report
Thu 26 May 2016
SRR1781358_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781358_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646571
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15640724.190228141998325No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAC252.3952038E-455.97654712
CGCACCT252.3961194E-455.9722188
AGCGCAT200.006938792352.522745
TGCCGGA200.006940916652.5186447
GCCGGAT200.00694304252.51457254
AGAACGT200.006949419652.5023862
CGGCACA200.006960059552.48207514
CGACGAG407.5008575E-752.47801622
CATCGTT305.862732E-446.6904642
CGCGGGT305.891876E-446.643527
TGCCGGC1300.045.753619
GGTCTCG551.3061799E-744.5681741
GTCCGCA404.5913326E-543.75198432
GACGCAC404.606034E-543.72836
CTGGCGC2050.040.9584512
ACGGTGA350.00125747139.9863415
CTACGGT350.001257949539.9832513
TGGCGCT2550.039.78725413
TTGTGCG459.203358E-538.8846322
GCCGGCG2250.038.8756120