FastQCFastQC Report
Thu 26 May 2016
SRR1781358_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781358_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646571
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT8066712.476124045155133No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG267664.139684582203656No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC65371.0110258579490883No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC58310.901834446642364No Hit
ATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCTT38840.6007074242426585Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGAGCCTTATCTCGTATGCCGT18670.2887540579456858No Hit
CTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC16210.2507071922495751No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTAATCTCGTATGCCGT12260.18961568025785258No Hit
TTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC11030.17059224740979723No Hit
CTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG9900.15311543511849435No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC9780.1512594904503914No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGACTTATCTCGTATGCCGT9190.1421344291655518No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCATTATCTCGTATGCCGT9080.14043314655312408No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGCCTTATCTCGTATGCCGT8940.13826787777367064No Hit
CATATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT8850.1368759192725934No Hit
TATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCT8060.12465761687424892No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGACGT7030.1087274251396985No Hit
CTTAAACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT6850.10594350813754405No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCCG150.002224960169.99605647
CGCTAGT150.002224960169.99605626
CTTACGT150.002224960169.99605632
AGGGGGG116800.063.06390470
GCCGACA700.059.99661646
GTAAGCG200.00694584152.50922460
AAGGCCG200.006952217352.49704415
CGACTAC750.051.3304448
TCGAAAG1100.050.90622340
TCTCGAA4000.049.87219238
TAACTCG3450.049.70734436
CGAAAGC1200.049.5805441
CTCGAAA1200.049.5805439
AACTCGA850.049.4089837
ATATCTC4400.049.31540335
TGCATTA2750.048.36091231
ACTCGAA950.047.8920438
TCCGACT305.8791175E-446.66403646
ATCCCGT305.8791175E-446.66403644
TTTATAC10600.046.557583
Produced by FastQC (version 0.11.5)