FastQCFastQC Report
Thu 26 May 2016
SRR1781357_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781357_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330170
Sequences flagged as poor quality0
Sequence length76
%GC77

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16456049.84099100463398No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5990.1814216918557107No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4530.13720204743011175No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4490.1359905503225611No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3380.1023715055880304No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3350.10146288275736741No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTTA150.00222152970.0156159
TATTTCG207.9233956E-569.9943919
CGTATAC150.002224201669.9943851
CGTAGAC150.002224201669.9943851
ACGCTGG150.002224201669.99438515
TAAGGTT150.002224201669.99438530
ACGAACG150.002224201669.99438524
GACGATT150.00222553969.9837923
CCCGGAT207.929346E-569.9837913
GTATGCG150.002228215369.9625852
ATGCACG207.947217E-569.9519964
CTTATGC750.065.32811
CGCGTCT1250.061.5577557
CGCGTGT401.0697477E-861.2079937
ATTCGAT352.9710645E-759.99519329
TTATGCG700.059.9679342
GCGCGTC1350.059.588736
ACGTCTG1000.059.4591948
CACGTCT950.058.906947
GCCGGAT308.355855E-658.3463454
Produced by FastQC (version 0.11.5)