FastQCFastQC Report
Thu 26 May 2016
SRR1781357_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781357_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330170
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT8515525.79125904836902No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG273808.292697701184238No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC65811.9932156161977164No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC61941.8760032710421906No Hit
ATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTT50121.5180058757609716RNA PCR Primer, Index 32 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCAATCATAATCTCGTATGCCGT21980.6657176605990853No Hit
CTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC17970.5442650755671321No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATCTTAATCTCGTATGCCGT15950.483084471635824No Hit
TTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC12940.3919193142926371No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC10590.3207438592240361No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTAAATCTCGTATGCCGT10510.3183208650089348No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAACTTAATCTCGTATGCCGT9970.30196565405700093No Hit
CTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG9950.3013599055032256No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATATCGTATGCCGT9830.29772541418057363No Hit
CATATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT9350.2831874488899658No Hit
TATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCT9170.27773571190598784No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTATCTCGTATGCCGTC8820.2671351122149196No Hit
CTTAAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT7960.2410879244025805No Hit
CTTATACACATCTCCGAGCCAACGAGACCAATCTTAATCTCGTATGCCGT7440.22533846200442198No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGACGT7040.2132234909289154No Hit
CCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG6940.2101947481600388No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGA6610.20019989702274588No Hit
CTTTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC6050.18323893751703668No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCAGT5920.17930157191749704No Hit
CTTATACACATCTCCGAGACCACGAGACCAATCTTAATCTCGTATGCCGT5810.17596995487173275No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAAACTCGTATGCCGT5640.17082109216464245No Hit
CTTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG5370.16264348668867554No Hit
TCTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT5250.15900899536602356No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGAATGCCGT5210.15779749825847292No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATCATAATCTCGTATGCCGT5130.15537450404337158No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTAAGCCGT4040.12236120786261623No Hit
CTTATACACATCACCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT3700.11206348244843566No Hit
CTTATACACATCTCCGAGCACACGAGACCAATCTTAATCTCGTATGCCGT3700.11206348244843566No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCAG3390.10267437986491808No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTCTTAATCTCGTATGCCGT3370.10206863131114274No Hit
CTTATACACATCTCAGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT3340.10116000848047976No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCACG150.002223476870.0001536
TCCATGG150.00222481369.98955514
GCGCACT150.00222481369.98955545
GACCCAT150.00222481369.98955526
CCCGTCA150.00222481369.98955546
CTCGGTA150.00222481369.98955526
CACTACG150.00222481369.98955548
ATCTTGA150.00222481369.98955555
CGCACTA150.00222481369.98955546
GCTCGGT150.00222481369.98955525
CCAGTAG150.00222481369.98955524
TGTCGGC150.00222481369.98955515
GCCAAGC150.00222481369.98955520
TACCGTC150.00222481369.98955545
GCCGACA700.064.990346
AGGGGGG127550.062.8393570
TCTTTAT8300.061.97871
AATCACG2900.061.5518636
CCGAAAT401.0652002E-861.24085247
CACGATA352.9725015E-759.99104721
Produced by FastQC (version 0.11.5)