FastQCFastQC Report
Thu 26 May 2016
SRR1781356_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781356_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647062
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14151021.869619912774972No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGGT150.002221619570.0225844
TGCGCGT850.053.5135735
GTTGCGA200.006941810752.51693336
GCGTATC200.006946057552.50881255
TTGACCG200.006956683552.4885222
TCGACTT200.006956683552.4885220
TTGTGCG1000.049.004442
TGTGCGC950.047.8805663
CGTATCA1050.046.674556
ACGTCGG305.8838236E-446.6564678
TCTGGCG1750.045.9863911
CGTCTGG1300.043.0675059
TGGCGCT2050.040.9634913
TGCCGGC2200.039.76403419
CTGGCGC2500.039.188412
GCCGGCG3350.037.60371820
GTGGTCG1500.037.3367148
GTGCGCG1500.037.3222854
CCGGCGA951.891749E-1036.83405321
GGCGCTG4050.035.42435514
Produced by FastQC (version 0.11.5)