FastQCFastQC Report
Thu 26 May 2016
SRR1781356_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781356_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647062
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT7456611.523779792353746No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG262534.057261900714305No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC67271.0396221691275334No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC41610.6430604795212824No Hit
ATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTCTT27520.4253070030383487RNA PCR Primer, Index 45 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGGTCAATTATCTCGTATGCCGT19420.3001257993824394No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATATATCTCGTATGCCGT13730.21218986743155988No Hit
CTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC10200.15763558978892284No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATTAATCTCGTATGCCGT9920.15330833830452106No Hit
CATATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT7660.11838123703756365No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT7230.11173581511508943No Hit
TTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC7060.10910855528527405No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC7050.10895401058940257No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG6900.10663584015133017No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACATTTATCTCGTATGCCGT6870.10617220606371569No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAATCG150.002224210270.00201436
AGGGGGG107900.064.5523670
CGGTAAT1350.054.441828
ATCGAAT452.7021088E-854.441839
CGGTCAA6650.054.20789328
TGTAGCG200.006952006452.497457
GCGTAGT200.006952006452.4974540
CACGTAT2300.051.73661839
ACGGTAA1650.050.9066227
CGACTAC900.050.55309748
ACCGTCA352.0870559E-549.9975727
GACGGTA1700.049.40936326
TATCACG2200.049.31959536
AACTCGA1000.048.9976237
ACTCGAA1150.048.69328738
GTCAATT6250.048.16138530
TTTATAC7450.047.4510273
GACGGAC5250.047.33103626
CGGACAT3950.046.95974328
ACGGACA5150.046.89092627
Produced by FastQC (version 0.11.5)