FastQCFastQC Report
Thu 26 May 2016
SRR1781355_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781355_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585344
Sequences flagged as poor quality0
Sequence length76
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24244641.41940465777389No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6690.11429176689263065No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACG207.9395184E-569.974792
CTTCGTG150.002229802369.956867
CGGCGTT207.949604E-569.9568625
ACGGCCT150.002229802369.9568624
TCTCCGC150.002229802369.956868
ACGACCC150.002229802369.9568624
GACGAGC252.8496652E-669.95685623
CGCGTCT600.064.127127
TGGCGAC308.405632E-658.29738219
GGTGCCG308.405632E-658.29738217
GGCGCAT252.3973553E-455.9654855
GCGTCGG252.3973553E-455.9654858
GTACGCG252.3973553E-455.9654854
TGGCGCT2150.055.31472813
GCGCGTC700.054.9661036
GTGCGCG653.6379788E-1253.8129654
TTGTGCG1050.053.3141252
TCGACGG200.006934408652.53046852
GTGGTCG2400.052.53046848
GGGGTCA200.00693910152.52148456
Produced by FastQC (version 0.11.5)