FastQCFastQC Report
Thu 26 May 2016
SRR1781355_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781355_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585344
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT14452224.690096763612505No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG168572.8798450142138643No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC93241.5929094686201617No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT86601.4794719002842773No Hit
CATATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT41270.7050554887382462No Hit
ATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCTT37070.6333028099715723TruSeq Adapter, Index 18 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC31470.537632571616007No Hit
ACTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG27690.4730551607260004No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACATCATCTCGTATGCCGT21900.3741389678547999No Hit
CTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC18480.3157117865733654No Hit
CTTAAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT16080.2747102558495517No Hit
CTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG12020.2053493330417669No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10120.17288978788541437No Hit
CAAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT10040.1715230701946206No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAACCTCATCTCGTATGCCGT9220.15751421386398426No Hit
CTTATACACATCTCCGAGCCCACGAGACTACAACTCATCTCGTATGCCGT8960.15307238136890444No Hit
TCATATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG8670.14811802973977695No Hit
CCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG8040.13735512792477586No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATATCGTATGCCGT7370.1259088672643779No Hit
CTTATACACATCTCCGAGCCAACGAGACTACACCTCATCTCGTATGCCGT6680.11412092718128142No Hit
CTTTACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC6510.11121665208834464No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG5890.10062458998469277No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG221200.065.4507970
AAATCGA453.8562575E-1062.2189637
CGTCATA453.8562575E-1062.2189648
TAGACTA850.061.76146724
CAAATCG1200.058.33027336
CGACTTC4300.057.78766648
CCGTCTA3800.057.10226447
ATGCCGA5200.056.53549244
CTAATAC24200.056.2766081
TCGTAAG5100.056.27155740
ACTCGTA4800.056.1428938
AAACTCG252.390684E-455.99706636
CGAATTC509.822543E-1055.99706648
TCTCGAA5750.055.99706338
CCGACTT3950.055.81985547
TGCCGAC4900.055.7113645
AAGGGGG259950.055.54610469
GACGACT1200.055.4137646
GAACACG1200.055.4137618
TGACGAC950.055.26025445
Produced by FastQC (version 0.11.5)