FastQCFastQC Report
Thu 26 May 2016
SRR1781354_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781354_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717439
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8711912.143053277003341No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGA200.00693181852.5366537
GATCGAT200.00693181852.5366538
TCGTGTC200.006937560652.5256549
GTCGCCT200.006970168652.46340621
GGTGTCG503.4623936E-642.0293241
TTGACGG459.202213E-538.88617731
CGTCTGG852.5975169E-937.027839
TGTCGGG501.7028426E-435.02443343
CATCGTT708.6891123E-735.0024432
TGCCGGC1102.3646862E-1134.97804319
CGATTGA708.741408E-734.97560521
CTGGCGC1350.033.67551812
CGGTAGG851.0581971E-732.9618736
TCTGGCG751.5005753E-632.63934711
TTGCGAT1001.2503733E-831.52198845
TCGGTGG1450.031.40121545
TGGCGCT1450.031.35962513
ACGCTAG901.7475031E-731.13065736
TAGGCGC450.004325063431.085093
CGGTGGT1251.05501385E-1030.82149946
Produced by FastQC (version 0.11.5)