SRR1781354_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1781354_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	717439
Sequences flagged as poor quality	0
Sequence length	76
%GC	43

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT	36539	5.092976545741171	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG	14081	1.9626755724180034	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC	3815	0.5317525253018027	No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCAGATCTCGTATGCCGT	3620	0.5045725141789058	RNA PCR Primer, Index 47 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCTT	3430	0.4780894264181345	Illumina PCR Primer Index 9 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC	2467	0.34386198687275155	No Hit
CTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC	1064	0.1483052914603193	RNA PCR Primer, Index 47 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACACTCGCAGATCTCGTATGCCG	975	0.13590005561448432	No Hit
TTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC	753	0.10495665833610941	RNA PCR Primer, Index 47 (95% over 22bp)
TCTTAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC	747	0.10412035030155874	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCATCG	15	0.002224791	69.998184	5
AGGGGGG	6500	0.0	59.142162	70
ACGGCTT	20	0.0069516897	52.49864	32
CACTACG	20	0.0069516897	52.49864	2
GCTAGCG	20	0.0069516897	52.49864	36
ATCGATC	20	0.0069516897	52.49864	6
ACGCAGA	180	0.0	48.60985	31
TATGACG	215	0.0	45.577034	43
TTTATAC	765	0.0	44.83544	3
CGTCTAG	90	1.8189894E-12	42.77667	1
CCGTCTA	215	0.0	42.321533	47
AAGGGGG	9300	0.0	41.29827	69
GATACCG	60	2.5999543E-7	40.82943	7
ATCTCGT	9510	0.0	40.295834	37
TATGCCG	9690	0.0	40.089046	43
GATCTCG	9620	0.0	40.056137	36
ACAATCG	70	1.9270374E-8	39.998966	27
ACCGATC	35	0.0012560434	39.996178	8
ATGCCGT	9595	0.0	39.902386	44
CTCGTAT	9750	0.0	39.87824	39