FastQCFastQC Report
Thu 26 May 2016
SRR1781353_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781353_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806347
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16428620.374106929150848No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA1250.061.61709656
CCGTATC1150.057.8457355
TTGTACG452.7175702E-854.4055252
CCGTCTA200.006953894652.4950230
GTCGACT200.006964138752.47548311
TTGTGCG1300.051.117272
TGTACGC552.2992026E-950.8726963
ATTCGAT352.0896214E-549.9890529
CGCCGTA1550.049.69120853
TCTCGGT2200.049.34718743
GTGGTCG3100.048.56789448
ACACGTC950.047.8634646
CTCGGTG2600.047.13723844
CGATGTG604.9276423E-946.67672332
CGCGTGT604.965841E-946.6361967
CTTATGC1200.043.7295721
GCCGTAT1550.042.91513454
TGCGCGT901.8189894E-1242.7471965
CGGTGGT3450.042.6284646
GGTCGCC2800.042.51179550
Produced by FastQC (version 0.11.5)