FastQCFastQC Report
Thu 26 May 2016
SRR1781353_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781353_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806347
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT8272510.259230827422932No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG281513.491176875464285No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC65300.8098250505055516No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC53600.6647262282863333No Hit
ATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCTT36500.45265871888901427Illumina PCR Primer Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAACGCCAAATCTCGTATGCCGT23860.29590238445731176No Hit
CTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC13510.16754573403261872No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGCCATATCTCGTATGCCGT12780.15849255965483844No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTAAGCCGT12430.15415199659699858No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCAATATCTCGTATGCCGT10300.12773656998785882No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGACATATCTCGTATGCCGT10290.12761255390049198No Hit
TTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC10120.12550428041525546No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCAGT9910.12289994258055156No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC9620.11930347604691281No Hit
CATATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT9400.11657512212484204No Hit
CTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG8500.10541367426182525No Hit
CTTAAACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT8190.1015691755534528No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGCCATATCTCGTATGCCGT8070.10008098250505057No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG124750.062.9640570
ATACCGT308.379153E-658.3317844
TGACGTA608.367351E-1152.49860445
CACGTAA1050.049.9955739
CAACGCA5750.049.91171628
ACGTAAG1000.048.9956640
TTTATAC10600.048.8666273
ATCGAAT651.8553692E-1048.45724539
TCTCGAA5400.047.95871438
AACGCAA6400.047.57686229
TCTTTAT10200.047.369311
CCGACTT3750.046.6654347
CGCAATA5850.046.66542431
CGACTTC4300.046.39411548
GCCAAAT8450.046.38929732
CGACATA3550.046.33679231
TAACTCG4050.045.8012536
TATGACG4850.045.46270843
CGCCAAA9600.045.2071331
GCAATAT5600.044.99880232
Produced by FastQC (version 0.11.5)