FastQCFastQC Report
Thu 26 May 2016
SRR1781352_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781352_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237748
Sequences flagged as poor quality0
Sequence length76
%GC94

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17215572.41070377037872No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5910.24858253276578562No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4570.19222033413530293No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4050.1703484361592947No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3670.1543651260999041No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3540.14889715160590206No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3470.14595285764759325No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3000.12618402678466276No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2900.12197789255850733No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2660.11188317041573431No Hit
GGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGG2480.10431212880865455No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2390.10052660800511466No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATG150.00221898970.029738
GTATGAT150.00221898970.029757
GAGCGCC150.00221898970.029750
GTCACAT207.8990764E-570.029757
AGGACCA150.00221898970.029739
GGACCAC150.00221898970.029740
CCTCGGT150.00221898970.029738
CGCTCGA150.00221898970.029750
TGTTTAC207.8990764E-570.029734
TTACAAC301.0177973E-770.029751
GAGCCGT207.8990764E-570.029745
TGTCACA207.8990764E-570.029756
ATCAGAG252.8269205E-670.029741
CCGTAGC150.00221898970.029755
CCACCCC150.00221898970.029743
TCTGTAG207.8990764E-570.029742
GCCGGTT150.00221898970.029759
CACCCCG150.00221898970.029744
AGAGCCG207.8990764E-570.029744
CTGTGTA301.0177973E-770.029734
Produced by FastQC (version 0.11.5)