FastQCFastQC Report
Thu 26 May 2016
SRR1781352_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781352_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237748
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT9119038.35573800831132No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG2974112.509463802008849No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC72843.0637481703316114No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC54002.2713124821239297No Hit
ATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCTT33711.4178878476369938RNA PCR Primer, Index 20 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAAAAGCCATCTCGTATGCCGT15910.6691959553813281No Hit
CTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC15470.6506889647862443No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGACATCTCGTATGCCGT15440.6494271245183977No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCAATCTCGTATGCCGT15310.6439591500243955No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGACGT10780.4534212695795548No Hit
TTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC10520.4424853205915507No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC10270.43196998502616213No Hit
CATATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT9860.4147248346989249No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGA9720.40883624678230734No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTAAGCCGT9280.3903292561872235No Hit
CTTAAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT9230.38822618907414574No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG8850.37224287901475517No Hit
CTTATACACATCTCCGAGCCAACGAGACGAAATGCCATCTCGTATGCCGT7620.3205074280330434No Hit
TATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCT7350.3091508656224237No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGAATGCCGT7350.3091508656224237No Hit
CCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG6950.292326328717802No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCAGT6850.2881201944916466No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCACGTATGCCGT5950.2502649864562478No Hit
CTTTACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC5840.24563823880747682No Hit
TCTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT5450.22923431532547067No Hit
CTTATACACATCTCCGAGCCCACGAGACGAACTGCCATCTCGTATGCCGT5160.21703652606961996No Hit
CTTATACACATCTCCGAGACCACGAGACGAAATGCCATCTCGTATGCCGT5070.21325100526608004No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCAACTCGTATGCCGT4800.2018944428554604No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATATCGTATGCCGT4670.19642646836145836No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCAATCTCGTATGCCG4630.19474401467099617No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAAAGACATCTCGTATGCCGT4480.18843481333176304No Hit
CTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG4220.17749886434375894No Hit
CTTATACACATCTCAGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT3720.1564681932129818No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCAG3700.15562696636775072No Hit
CTTATACACATCACCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT3690.15520635294513518No Hit
TCTTATACACATCTCCGAGCCAACGAGACGAAATGCCATCTCGTATGCCG3430.1442704039571311No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTAAGCCG3360.14132610999882228No Hit
CTTATACACATCTCCGAGCACACGAGACGAAATGCCATCTCGTATGCCGT3250.1366993623500513No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGAAATCTCGTATGCCGT3170.13333445496912696No Hit
CTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT3060.12870770732035602No Hit
TCCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC2830.11903359860019853No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGACG2480.10431212880865455No Hit
CTTATACACAACTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT2480.10431212880865455No Hit
GCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG2460.10347090196342346No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGACA150.00221997370.0218969
AGGGGCG252.8288014E-670.0218970
GCCCAGA150.00221997370.0218969
ATAAGGG150.00221997370.0218968
AGAGGGT150.00221997370.0218970
AGCGGGG750.070.0218970
GTGACAA150.00221997370.0218970
ACGTGAC150.00221997370.0218970
AGGGGGA207.903454E-570.0218870
AGTGGGG550.070.0218870
ATGTGAC207.903454E-570.0218868
CATGTGA207.903454E-570.0218867
CTTTTAT150.002221826770.007161
TCCCATC600.070.0071633
CTGAGTG207.911697E-570.0071629
CATTGCG150.002221826770.0071629
GGGTTAT150.002221826770.0071628
TAAATGA150.002221826770.0071629
TGCGCTG150.002221826770.0071632
CTTTACA600.070.007161
Produced by FastQC (version 0.11.5)