FastQCFastQC Report
Thu 26 May 2016
SRR1781351_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781351_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549319
Sequences flagged as poor quality0
Sequence length76
%GC75

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21772739.635803604098896No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8520.15510113431357736No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6060.11031841243430503No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5500.10012397168129993No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGTG150.002223927370.0028330
GTGCTTA150.002227142769.977339
TTATGCG453.8562575E-1062.202072
CTGTACT308.39944E-658.3038254
CGTTAGA252.3883814E-456.00736661
GTTATGC509.804353E-1055.9971581
TTTTACG252.3926848E-455.9869625
CTAGATA252.3937615E-455.9818652
CTTATGC653.6379788E-1253.8434221
TCATTAA2100.053.34034360
TACCTCT200.00693649652.5260439
CGTATCA2600.052.506956
TTAGAGT200.00694650152.506961
CGTCTTA200.00694650152.506961
GGTACTA200.006956516352.48777821
GGGACTC200.006956516352.48777824
TCTCGGT3550.052.27943843
TAGATCT2850.050.3758539
GTATCAT2450.050.00657357
AGATCTC3400.049.43627540
Produced by FastQC (version 0.11.5)