FastQCFastQC Report
Thu 26 May 2016
SRR1781351_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781351_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549319
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT11666821.238660960207092No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG361506.580875593234532No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC80101.4581691148494773No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC78591.4306805335333386No Hit
ATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCTT52410.9540904283303508No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGAATTATCTCGTATGCCGT37510.6828454868664655No Hit
CTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC24520.4463708701137227No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATAATCTCGTATGCCGT21800.39685501502769793No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAAATATCTCGTATGCCGT20780.3782865693704387No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC15930.28999543070601963No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC14610.26596567750250766No Hit
CTTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG14420.26250684938988095No Hit
CATATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT13670.24885358052424913No Hit
CTTAAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT12080.2199086505291097No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGACGT11960.21772412751060857No Hit
TATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCT10610.19314824355247132No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGA10160.18495628223309224No Hit
CTTATACACATCTCCGAGCCAACGAGACCAAGAATTATCTCGTATGCCGT9530.17348753638596154No Hit
CCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG8830.16074448544470518No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCAGT8580.1561933958228279No Hit
CTTTACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC8550.15564726506820262No Hit
TCTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT8100.14745530374882354No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTAAGCCGT7990.14545282431519754No Hit
CTTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG7580.1379890373353188No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTAACTCGTATGCCGT7400.13471225280756718No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGAATGCCGT7350.13380203488319176No Hit
CTTATACACATCTCCGAGACCACGAGACCAAGAATTATCTCGTATGCCGT7340.13361999129831664No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGAATAATCTCGTATGCCGT6910.12579211714868774No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTGTA207.924377E-570.0013
AGGGGGG162400.064.62475670
TCTTTAT9950.061.212481
CATTATC850.057.64788433
CTTAAAC7000.055.00581
TAACTCG4850.054.12448536
ATGTTAC200.00694221952.5150967
TTAACTC3600.052.5007535
TTTATAC12200.052.5007483
TCCTAAG200.006952224852.4959740
TGCATTA407.481103E-752.4959755
TGCTAAC200.006952224852.4959710
ATATCCG2500.051.7960310
CTTTATA12150.050.9883842
TTAAACA8050.050.4355052
ACTCGAA1600.050.3086438
TCGAATG7100.049.78491240
CACGTAT3800.049.7330339
TCGTATC850.049.4079740
CGAGACA14800.048.94894423
Produced by FastQC (version 0.11.5)