FastQCFastQC Report
Thu 26 May 2016
SRR1781350_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781350_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653304
Sequences flagged as poor quality0
Sequence length76
%GC94

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50107476.69844360359036No Hit
CTTATACACATCTGACGCTGCCGACGAAGATCTCTGTGTAGATCTCGGTG47790.731512435252195No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17980.275216438289066No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11280.17266081334263989No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11130.1703647918886154No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10010.1532211650318994No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8900.13623060627211836No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8640.13225083575180926No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7640.11694402605831283No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTC150.002218388570.04835539
TAGATGT353.6907295E-970.04835539
TCCCCGT150.002218388570.04835538
TCGTTTA150.002221086870.0268954
GAGACAA150.002221086870.0268954
GATTCGT150.002221086870.0268951
TTGATTC150.002221761770.0215249
TGATTCG150.002221761770.0215250
CATGTCT150.002221761770.0215256
CCTACGT207.916997E-570.0161657
GTTATGC455.456968E-1270.005441
TCGGGAA207.923007E-570.0054360
TTCTTCC150.00222446370.0000864
CATGGGT150.00222513969.9947232
GAACTCT150.002225814969.9893629
CGTACCT150.002225814969.9893629
TTATGCA550.069.9842
TTCTAGG150.00222716769.9786428
CAACCCT150.002227843369.9732816
CCATCCC150.002227843369.9732817