FastQCFastQC Report
Thu 26 May 2016
SRR1781350_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781350_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653304
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT37478057.36686136928597No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG437506.696729240904693No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC197683.025850140210377No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77961.1933188837049826No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC76281.1676034434199087No Hit
CATATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT59460.9101429043752985No Hit
CTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT52870.8092710284951569No Hit
ATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCTT48950.7492683344966509RNA PCR Primer, Index 17 (95% over 22bp)
CTTAAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT35220.5391058374049448No Hit
CTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC31070.4755825771769345No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGCTCTCGTATGCCGT27880.4267538542546808No Hit
CTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG25990.3978239839339725No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAAACAGATCTCGTATGCCGT23110.3537403720167028No Hit
ATTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT19370.2964929037630261No Hit
CCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG16500.2525623599426913No Hit
CTTTACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC15920.24368441032046337No Hit
CTTATACACATCTCCGAGCCCACGAGACAACTACAGATCTCGTATGCCGT14690.22485703439746274No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC13530.20710113515300688No Hit
CTTATACACATCTCCGAGCCAACGAGACAAATACAGATCTCGTATGCCGT12810.19608023217368942No Hit
CTTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG12080.18490626109743702No Hit
TTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC11930.18261023964341255No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGACGT10650.16301752323573712No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGCTCTCGTATGCCG10560.16163991036332243No Hit
CTTATACACATCTCCGAGACCACGAGACAAATACAGATCTCGTATGCCGT10380.1588846846184931No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCAGT9520.14572082828208613No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATAAAGATCTCGTATGCCGT9450.1446493516035414No Hit
TATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCT9120.13959810440468756RNA PCR Primer, Index 17 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATATCGTATGCCGT8660.1325569719456792No Hit
CTTATACACATCTCCGAGCACACGAGACAAATACAGATCTCGTATGCCGT8070.1235259542265163No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGA7740.11847470702766247No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAATCTCGTATGCCGTC7730.1183216389307275No Hit
CTTATACACATCTCAGAGCCCACGAGACAAATACAGATCTCGTATGCCGT7680.11755629844605267No Hit
TCATATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG7270.11128050647171914No Hit
CAAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT7230.11066823408397929No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGCT150.002222997870.0117163
ACGCATC500.070.0063556
TATACGC455.456968E-1270.0063553
TATTGAA207.925501E-570.0009956
AACGTAA150.002225023569.99563639
ATGATTG301.0240365E-769.99563654
CTACTTG301.0240365E-769.99563654
GATAAAG353.7125574E-969.99563636
AGTCAAC252.8406703E-669.99563648
TAAAGTA353.7125574E-969.99563638
TACCAAG150.002225023569.99563618
CGAAACC150.002225023569.99563641
GCGAATG150.002225023569.99563638
CATGAGC150.002225023569.99563634
GGCAAAT150.002225023569.99563626
GATCCAA301.0240365E-769.99563616
GCCGGTA150.002225023569.99563617
CACGCAT150.002225023569.99563645
GCACGCA150.002225023569.99563644
CTCCAAG150.002225023569.99563654
Produced by FastQC (version 0.11.5)