FastQCFastQC Report
Thu 26 May 2016
SRR1781349_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781349_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1301002
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47082936.18972146084326No Hit
CTTATACACATCTGACGCTGCCGACGAAGATCTCTGTGTAGATCTCGGTG70810.5442727989657203No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTCG150.00222333270.01369
ACTCGTT207.950435E-569.9618722
TTGTGCG2000.057.716322
CGGTGGT14350.054.1793246
CCGTATC12350.054.15002455
CGTATCA12650.053.9729756
CGCCGTA12500.053.7782653
TCTCGGT13850.053.61076743
TCGCCGT12900.053.46776652
GTGGTCG14250.053.32250648
GCCGTAT12700.052.93339554
ATCTCGG13250.052.60210842
TTCGGTA200.00696829852.4693832
CTCGGTG14500.052.41323544
GATCTCG13450.052.08032241
TCGGTGG15800.051.8670245
TGGTCGC14250.051.35274549
GGTCGCC13850.051.3150950
GTCGCCG14150.050.22906551
CGTCTGG2950.049.8033649
Produced by FastQC (version 0.11.5)