FastQCFastQC Report
Thu 26 May 2016
SRR1781349_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781349_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1301002
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT32570925.035242067268154No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG386192.9684043529525703No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC174571.3418119264997288No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCCCAGCATCTCGTATGCCGT139261.0704057334270047No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100790.7747105692381717No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC71350.5484234459285997No Hit
ATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTCTT39860.306379236926615RNA PCR Primer, Index 43 (95% over 22bp)
CATATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT36550.2809373083208173No Hit
CTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT32800.25211337107859944No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACAAGCATCTCGTATGCCGT28540.21936937837143985No Hit
CTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC26810.20607193532369666No Hit
CTTAAACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT23200.17832409173852154No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTAAGCCGT23060.17724799808147873No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG21520.1654109678540079No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGCCCAGCATCTCGTATGCCG17640.1355878007873931No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTAGTATGCCGT16600.127593962192218No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGACGT14760.11345101698536973No Hit
ATTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT13650.10491913156167323No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG409950.067.32310570
CGACATA553.6379788E-1157.26791448
CCGTCTT466750.056.01029247
GCCGTCT470200.056.00869446
AAGGGGG492950.055.9663369
CGTCTTC463500.055.89713748
GTCTTCT448650.055.84396449
CTGCTTG447400.055.83786854
ATGCCGT495550.055.80602644
CTCGTAT495400.055.79681439
TGCCGTC488300.055.79604745
TTCTGCT435250.055.778252
TATGCCG495850.055.77226343
TCTCGTA500850.055.76265738
TGCTTGA457000.055.76003655
ATCTCGT500700.055.74440837
CTTCTGC449700.055.7213651
GTATGCC497450.055.65619742
TCGTATG499200.055.57325440
TCTGCTT448050.055.55376453