FastQCFastQC Report
Thu 26 May 2016
SRR1781348_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781348_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022848
Sequences flagged as poor quality0
Sequence length76
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44922743.91923335627581No Hit
CTTATACACATCTGACGCTGCCGACGAAGATCTCTGTGTAGATCTCGGTG43890.42909601426604926No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11420.11164904267300714No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAC150.0022207670.0323635
TTGTACG207.9350335E-569.9878162
ACGCGTC453.8744474E-1062.1779446
TTATGCG650.059.2204672
TTGTGCG2150.056.9668272
CGCGTCT2300.056.264287
GGATACG252.392968E-455.9902572
TGTGCGC2250.055.960153
GATACGC252.3993364E-455.9601483
TATGCGC653.6379788E-1253.8078353
CGTCTGG3300.052.9977469
TTATCGG200.006939127652.5242655
ATTCGAT200.006957949652.48829729
TTCGCGC200.00696872252.4677710
GTACGCG407.5203934E-752.462644
TGGCGCT5250.051.30934513
CCGTATC8200.050.81615455
CGCCGTA8550.050.3716853
TCGCCGT8850.050.24428652
CGTATCA8450.050.136656
Produced by FastQC (version 0.11.5)