FastQCFastQC Report
Thu 26 May 2016
SRR1781348_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781348_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022848
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT33063732.32513530847203No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG407143.9804545738956323No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC199081.9463302465273433No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69700.6814306720060068No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC68310.6678411650606932No Hit
CATATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT45650.4463028719809786No Hit
CTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT41860.4092494681516706No Hit
ATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCTT39910.3901850519334251RNA PCR Primer, Index 24 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT28590.27951367163058444No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTAAGTATCTCGTATGCCGT27230.26621746339632085No Hit
CTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC22510.220071799524465No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGAATCTCGTATGCCGT20440.19983418846201978No Hit
CTTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG18530.1811608371918408No Hit
ATTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT15560.15212426479789765No Hit
CCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG15350.15007117382054813No Hit
CTTATACACATCTCCGAGCCCACGAGACGACATAGTATCTCGTATGCCGT15020.14684488799899886No Hit
CTTTACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC12320.12044800400450507No Hit
CTTATACACATCTCCGAGCCAACGAGACGACTTAGTATCTCGTATGCCGT11430.11174680891002378No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC11290.11037808159179076No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGACGT10360.1012858215492429No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTAG150.002221452370.0268551
CGCTATA150.002225764669.99261515
AGGGGGG403450.067.5175370
ACTCGTA4500.062.2156638
TAACTCG4650.058.70348736
TCTCGAA6450.058.0558938
AAGGGGG468600.058.04831369
AAGTATC9250.057.88578433
TCGAAAG1700.057.64098440
GACAACG1600.056.86900318
TAAATCG800.056.86900336
CCGTCTA3850.056.35769347
ATGACGT6500.055.99409544
AGGTTCG252.3921583E-455.9940921
TATGACG6700.055.8896343
CTGCTTG479900.055.61926754
TGCTTGA481700.055.61757355
CCGTCTT478150.055.61057347
CGTCTTC481750.055.57275848
GCCGTCT481600.055.5682746
Produced by FastQC (version 0.11.5)