FastQCFastQC Report
Thu 26 May 2016
SRR1781347_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781347_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530595
Sequences flagged as poor quality0
Sequence length76
%GC94

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41763878.71125811588877No Hit
CTTATACACATCTGACGCTGCCGACGAAGATCTCTGTGTAGATCTCGGTG36730.6922417286254111No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11370.21428773358211067No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8790.1656630763576739No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6960.13117349390778277No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6770.12759260829823124No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6530.12306938437037665No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5880.11081898623243717No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5790.10912277725949171No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGTA150.00221727370.055443
AGGTTGG150.00221727370.055445
ACAGCTT150.00221727370.055444
CGATAAC150.00221727370.055437
CGACGTA150.002218933570.04217553
CCCCGAA207.901073E-570.04217553
CGTAGCA455.456968E-1270.03557656
GACGTAT150.002219764370.0355754
CCTAAGC150.002219764370.0355734
CCGAATC401.3460522E-1070.0355755
GCTACTA150.002219764370.0355752
TCCCCGA207.904767E-570.0355752
TAGTCTG150.002219764370.0355754
GCGTATC207.904767E-570.0355755
CCCGAAT207.904767E-570.0355754
GTAGCAG207.9084624E-570.0289657
GTATCGT150.00222059570.0289657
GCAGTAA150.002221426270.02235460
TCGGTAG207.9121586E-570.02235460
CAGTAAA455.456968E-1270.01575561