FastQCFastQC Report
Thu 26 May 2016
SRR1781347_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781347_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530595
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT29981856.50599798339599No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG379037.143489855728004No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC157232.9632770757357307No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC67391.2700835854088335No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61731.1634108877769298No Hit
ATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCTT44950.8471621481544305RNA PCR Primer, Index 45 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT37990.7159886542466476No Hit
CTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT34890.6575636785118594No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTATCTCGTATGCCGTC33720.6355129618635683No Hit
CTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC26440.4983085027186461No Hit
CTTAAACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT23820.44892997483956687No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG21120.39804370565120295No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTAATAATCTCGTATGCCGT20060.3780661333031785No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAATTAATCTCGTATGCCGT19750.3722236357296997No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATAATCTCGTATGCCGTC15720.2962711672744749RNA PCR Primer, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATATCGTATGCCGT14980.2823245601635899No Hit
CTTATACACATCTCCGAGCCCACGAGACTCATATTAATCTCGTATGCCGT14740.27780133623573533No Hit
ATTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT14700.27704746558109294No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG14290.26932029137100805No Hit
CTTTACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC13350.25160433098691093No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC11500.21673781320969854No Hit
TTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC11190.21089531563621972No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATAAATCTCGTATGCCGT10060.18959846964257107No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG10000.18846766366060744No Hit
CTTATACACATCTCCGAGCCAACGAGACTCTTATTAATCTCGTATGCCGT9710.1830021014144498No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGACGT8650.16302452906642545No Hit
TATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCT7660.1443662303640253No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCAGT7470.14078534475447374No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGA7400.1394660711088495No Hit
CTTATACACATCTCCGAGACCACGAGACTCTTATTAATCTCGTATGCCGT7260.136827523817601No Hit
CTTATACACATCTCAGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT5700.10742656828654623No Hit
TCATATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG5500.10365721501333408No Hit
CTTATACACATCTCCGAGCACACGAGACTCTTATTAATCTCGTATGCCGT5310.10007632940378255No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGCG150.002222959470.01018560
AGGGGTG252.836432E-670.01018570
CGTCAGC150.002222959470.0101851
CGCTTTC150.002222959470.01018569
TTAGGAG150.002223790670.0035864
GAGCTAG150.002223790670.0035865
ACGCATC301.0228541E-770.0035866
CCTAAAC150.002223790670.0035863
TTATACG301.0228541E-770.0035862
CCGGTCA207.922681E-570.0035864
ATGTTAC150.002223790670.0035865
CTTGTGA150.00222462269.9969925
AACAACG252.8396098E-669.9969910
GAAGCCG207.92638E-569.9969941
TGCCCAT150.00222462269.9969939
ACTGCCC150.00222462269.9969937
CAAGTGA150.00222462269.9969921
CGAAAGA150.00222462269.9969941
TGAAGCA150.00222462269.9969916
CCCTATA150.00222462269.9969945
Produced by FastQC (version 0.11.5)