FastQCFastQC Report
Thu 26 May 2016
SRR1781346_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781346_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517743
Sequences flagged as poor quality0
Sequence length76
%GC95

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41302379.77374875179383No Hit
CTTATACACATCTGACGCTGCCGACGAAGATCTCTGTGTAGATCTCGGTG35810.6916558987760337No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8370.1616632190102039No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7830.1512333339127714No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6690.1292146875959694No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5900.1139561519904663No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5520.10661660321819898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG252.828765E-670.0416839
CGCTATT150.002219817170.03490435
TCCATAA150.002221520370.0213749
CGTATAA150.002221520370.0213756
ATTACAC150.002221520370.0213752
GGAGTAA150.002221520370.0213760
TGACTTC150.002221520370.0213759
CTGGTGG207.912533E-570.021361
CTTATGC500.070.021361
CCTCCGA150.002222372270.01459566
TGCGTAC150.002222372270.01459565
GTAGACG150.002226635669.9807742
GATCTCA353.7161954E-969.98077429
TTATACG252.8434206E-669.980772
TACGCGT301.0264921E-769.9672555
TACACGT207.942892E-569.9672555
GACGCGT252.8466875E-669.967255
TAGAGAC207.946693E-569.9604953
GTACACA150.002229196769.9604954
GTCTGAC207.946693E-569.96049510
Produced by FastQC (version 0.11.5)