FastQCFastQC Report
Thu 26 May 2016
SRR1781346_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781346_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517743
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT30730459.354544629285186No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG379177.323517652580527No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC161713.1233642946403912No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC62201.201368246407967No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57491.1103964708359166No Hit
CATATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT41550.8025217144413349No Hit
ATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCTT36090.6970639873450727Illumina PCR Primer Index 10 (95% over 21bp)
CTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT34400.6644223099105154No Hit
CTTATACACATCTCCGAGCCCACGAGACTGAGCCTTATCTCGTATGCCGT23720.4581423602057391No Hit
CTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC22440.43341966960441763No Hit
CTTAAACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT22170.4282047270557014No Hit
CTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG17880.3453450843372098No Hit
CCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG14810.2860492560981027No Hit
ATTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT14760.28508352599648856No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTAATCTCGTATGCCGT14140.27310847273647354No Hit
CTTTACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC11800.2279123039809326No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGACTTATCTCGTATGCCGT11520.22250421541189355No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCATTATCTCGTATGCCGT11170.21574410470059469No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC10030.19372545838379274No Hit
TTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC9760.18851051583507647No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGCCTTATCTCGTATGCCGT8960.17305883420925053No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG8940.1726725421686049No Hit
CTTATACACATCTCCGAGCCAACGAGACTGTGCCTTATCTCGTATGCCGT8550.16513984737601473No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGACGT8070.15586883840051918No Hit
CTTATACACATCTCCGAGACCACGAGACTGTGCCTTATCTCGTATGCCGT7790.15046074983148008No Hit
TATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCT6930.1338501920837172No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCAGT6510.12573805923015857No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCATATCTCGTATGCCGT5710.11028637760433266No Hit
CTTATACACATCTCAGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT5600.10816177138078158No Hit
TCATATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG5530.10680974923852182No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGATT150.002223213770.0079262
GCGAAAA150.002223213770.0079266
ACCCTAG150.002223213770.0079262
TTATACG353.7052814E-970.007922
CTGCGAA150.002223213770.0079264
TACGCAT353.7052814E-970.007925
ATAAGGG353.7052814E-970.0079267
CTACCAT150.002223213770.0079267
AAGGGGA252.8368795E-670.0079269
GGATTCG150.002223213770.0079264
ATACGCA353.7052814E-970.007924
GTACCTT207.923857E-570.0011630
ACACCCT150.002224065670.0011630
GCTTGAC150.002224065670.0011656
TCAGATT150.002224065670.0011655
TTGGTCA150.002224065670.001169
TGCCTGA301.0230542E-770.0011631
CAGTGAC207.923857E-570.0011628
TACTCTA150.002224065670.0011632
TTGCCTT150.002224065670.0011630
Produced by FastQC (version 0.11.5)