FastQCFastQC Report
Thu 26 May 2016
SRR1781345_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781345_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577647
Sequences flagged as poor quality0
Sequence length76
%GC96

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46713780.86893898869032No Hit
CTTATACACATCTGACGCTGCCGACGAAGATCTCTGTGTAGATCTCGGTG46130.7985846027071897No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13170.22799391323766938No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10130.1753666166361117No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8750.15147659383672035No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8400.14541753008325153No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7880.1364154925066693No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6540.11321793413624584No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6440.11148677306382618No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTGT150.002220970670.0267746
CAGCAAA150.002220970670.0267738
AAAACGT150.002220970670.0267743
GTGGATC252.8327595E-670.0267738
AAACGTT150.002220970670.0267744
ATCCGCG150.002220970670.0267738
ATTCCAT207.910283E-570.0267736
TTCCATC207.910283E-570.0267737
TTGCGAC150.002220970670.0267740
TTTGCGA150.002220970670.0267739
CGATGGA150.002220970670.0267746
GCGCTTT150.002220970670.0267735
CGCTTTG150.002220970670.0267736
GGTAGAT207.910283E-570.0267737
CCCCGTT150.00222173470.02070647
TGGAGTC150.002222497770.0146455
CGGCGTA353.7034624E-970.0146453
GTCGCAG150.002222497770.0146451
CCAGGGG150.002222497770.0146433
GGGTATC207.917075E-570.0146455