FastQCFastQC Report
Thu 26 May 2016
SRR1781345_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781345_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577647
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT34030458.91210375887004No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG421217.291823553138855No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC180423.1233608068595524No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74641.2921386244540352No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC73541.2730958526574188No Hit
CATATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT44710.7740021154788305No Hit
ATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTT44350.7677699356181197RNA PCR Primer, Index 32 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCAATCTTATCTCGTATGCCGTC41330.715488871231046No Hit
CTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT40230.6964460994344297No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCATAATCTCGTATGCCGT28250.4890530029585543No Hit
CTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC27610.47797357209506847No Hit
CTTAAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT25740.4456008600408208No Hit
CTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG21340.3694297728543557No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATCTTAATCTCGTATGCCGT20790.35990838695604754No Hit
ATTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT15530.2688493145467734No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATATCGTATGCCGT14820.2565580709325938No Hit
CCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG14480.2506721232863669No Hit
CTTTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC14140.24478617564014007No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTAAATCTCGTATGCCGT13990.24218943403151058No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC12000.20773932869035933No Hit
CTTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG11660.2018533810441325No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAACTTAATCTCGTATGCCGT11480.1987372911137771No Hit
TTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC11290.19544808507617975No Hit
CTTATACACATCTCCGAGCCAACGAGACCAATCTTAATCTCGTATGCCGT10300.1783095904592251No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTCTTAATCTCGTATGCCGT10140.17553973274335363No Hit
CTTATACACATCTCCGAGACCACGAGACCAATCTTAATCTCGTATGCCGT8290.1435132529035899No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGACGT8230.1424745562601381No Hit
TATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCT7900.13676172472115322No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGA7140.1236049005707638No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCAGT6940.12014257842592449No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTAATCTCGTATGCCGTC6380.11044807642037438No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAAACTCGTATGCCGT6230.10785133481174489No Hit
CTTATACACATCTCAGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT6170.10681263816829309No Hit
TCTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT6160.10663952206105114No Hit
TCATATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG6120.10594705763208327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTG401.3460522E-1070.0219170
AGGGGGC500.070.0219170
AGCCAGA150.002221582770.0219164
TAACGAC150.002221582770.0219170
CCAGATT150.002221582770.0219166
ATGTGGG207.9130055E-570.0219170
GCCAGAT150.002221582770.0219165
AGCGGGG600.070.0219170
TCATGGG150.002221582770.0219166
CATGGGT150.002221582770.0219167
TACTTTC150.002221582770.0219161
GCATAAC150.002221582770.0219167
TGGACCG150.002223109470.0097858
CTTTACA1700.070.003731
CGTTTAC150.00222387370.0037238
TGCATTT150.00222387370.0037234
TGGTCGT150.00222387370.0037234
GTATGAT207.923195E-570.0037227
ACACGAA150.00222387370.0037238
CCAATGC150.00222387370.0037241
Produced by FastQC (version 0.11.5)