FastQCFastQC Report
Thu 26 May 2016
SRR1781344_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781344_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1291896
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44010834.06682890882857No Hit
CTTATACACATCTGACGCTGCCGACGAAGATCTCTGTGTAGATCTCGGTG42310.32750314266783087No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG150.002230699269.95464
TATGCGC850.057.6074333
TTGTGCG2050.054.6071622
TTATGCG1050.053.306992
CGTATCA8250.052.62489756
TTCGGTA200.00694558452.512760
TTGTACG200.006965825352.4740682
ACGCGTC407.5166645E-752.4679766
CCGTATC8000.052.08114655
TGCGCGT2350.050.6074075
CGCCGTA8650.050.19138353
CGGTGGT9900.049.87406546
GTCGCCG9700.049.8116551
CGTCTGG2950.049.8020449
TCGCCGT8800.049.73371552
GGTCGCC9800.049.29763850
TCTCGGT9850.048.71651543
GCCGTAT8600.048.4475854
ATCTCGG9350.048.3247942
GATCTCG9350.048.3247941
Produced by FastQC (version 0.11.5)