FastQCFastQC Report
Thu 26 May 2016
SRR1781344_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781344_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1291896
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT32351325.041721624650904No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG410153.1747911596599105No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC198101.5334051657408956No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67190.52008830432171No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC61970.47968257506796214No Hit
CATATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT40230.31140277545560946No Hit
ATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTCTT39030.30211410206394324RNA PCR Primer, Index 45 (95% over 23bp)
CTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT38100.2949153801854019No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT26470.20489265389783698No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCAATTATCTCGTATGCCGT24660.19088223819874045No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATTATCTCGTATGCCGTC22590.17485927659811626No Hit
CTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC22190.17176305213422752No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATATATCTCGTATGCCGT18510.14327778706645117No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG17780.13762717741985425No Hit
ATTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT15150.1172695015697858No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG15000.11610841739582753No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATTAATCTCGTATGCCGT13760.10651012155777244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG393550.067.1110170
TAACTCG3800.058.94365736
CCGTCTA3900.058.3251447
AAGGGGG455750.057.80585569
CGGTAAT2150.056.97756228
GCCGTCT473750.055.1574246
TGCCGTC475800.055.11836645
CCGTCTT470350.055.09484547
TATGCCG478150.055.05971543
ATGCCGT477800.055.0048344
CTCGTAT481900.054.9964839
TCTCGTA482200.054.95713438
CGTCTTC474700.054.95120648
ATCTCGT482150.054.94831537
CTGCTTG472900.054.93095854
TGCTTGA475500.054.86355
GTATGCC479950.054.8605142
TATCTCG480250.054.85963436
GTCTTCT477100.054.7554649
GCTTGAA480650.054.71932656