FastQCFastQC Report
Thu 26 May 2016
SRR1781343_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781343_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378958
Sequences flagged as poor quality0
Sequence length76
%GC94

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29173976.9845207120578No Hit
CTTATACACATCTGACGCTGCCGACGAAGATCTCTGTGTAGATCTCGGTG44791.1819251737659582No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7740.20424426981354135No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5380.14196823922439952No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5310.14012106882556907No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5240.13827389842673857No Hit
TCTTATACACATCTGACGCTGCCGACGAAGATCTCTGTGTAGATCTCGGT5230.13801001694119136No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4600.12138548335171707No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4470.11795502403960333No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3970.10476094976224277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTGC500.070.065671
TGTGAGC150.002220142670.02865642
GGAAATT150.002220142670.02865641
AATTCAT150.002220142670.02865644
CCTATTG150.002220142670.02865644
AGATCCT150.002221305670.0194239
TAGGTCG150.002221305670.0194239
AAGATCC150.002221305670.0194238
TAAGATC150.002221305670.0194237
GGAGCAT150.002223633270.0009357
CGAAACA150.002223633270.0009356
TGAACTA150.002223633270.0009356
GCGCCGT150.002223633270.0009352
TTATACG150.002223633270.000932
CTACGTT150.002223633270.0009363
ACTACGT150.002223633270.0009362
AGTCGCT150.002223633270.0009351
GTTAAAA207.921251E-570.0009262
TTGATGG150.002224797669.99168424
CGACGAT150.002224797669.99168422
Produced by FastQC (version 0.11.5)