FastQCFastQC Report
Thu 26 May 2016
SRR1781343_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781343_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378958
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT16046742.344270341304316No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT346349.139271370442106No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC122123.222520701502541No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG119993.1663139450809856No Hit
CATATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT90722.3939328368842987No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55411.4621673114170963No Hit
CAAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT42481.1209685506045526No Hit
ACTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG41491.0948442835353784No Hit
ATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCTT26740.7056190923532423TruSeq Adapter, Index 18 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT19670.5190548820713641No Hit
CTAAAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT17200.45387615514120294No Hit
TCATATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG16970.44780688097361715No Hit
CTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC15990.42194649538999046No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC15070.397669398719647No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACATCATCTCGTATGCCGT14120.372600657592662No Hit
ACATATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG13040.34410145715356316No Hit
CTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG9110.24039603333350928No Hit
CCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG7370.19448065484829455No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7340.1936890103916529No Hit
ACTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC6660.17574506937444254No Hit
CATAAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT6400.16888415075021507No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACATCATCTCGTATGCCGT6360.16782862480802624No Hit
CAAAAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT5240.13827389842673857No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAACCTCATCTCGTATGCCGT5230.13801001694119136No Hit
CTTATACACATCTCCGAGACCACGAGACTACACCTCATCTCGTATGCCGT5180.13669060951345532No Hit
CTTATACACATCTCCGAGCCAACGAGACTACACCTCATCTCGTATGCCGT5100.13457955762907764No Hit
CTTATACACATCTCCGAGCCCACGAGACTACAACTCATCTCGTATGCCGT4970.1311490983169639No Hit
CTTTACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC4890.12903804643258618No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATATCGTATGCCGT4870.12851028346149176No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACCTCATATCGTATGCCGT4800.1266631130626613No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG4770.12587146860601967No Hit
CTAATACACATCTCCGAGCCAACGAGACTACACCTCATCTCGTATGCCGT4630.12217712780835871No Hit
CTAATACACATCTCCGAGACCACGAGACTACACCTCATCTCGTATGCCGT4620.1219132463228115No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACCTCAACTCGTATGCCGT4350.11478844621303681No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCCCTCATCTCGTATGCCGT4330.11426068324194237No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGAATGCCGT4090.10792752758880932No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGACGT3910.10317766084895952No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGACGT3840.10133049045012903No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTAAGCCGT3800.10027496450794018No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG150.002221855670.01504560
AGTCCAG150.002221855670.01504565
GGGCCTA150.002221855670.01504567
CTTGTAA150.00222301970.00580657
ATGTGGG150.00222301970.00580670
CCATATA301.0218537E-770.0058061
TCATGAA301.0218537E-770.00580656
GCATCAA353.7016434E-970.00580656
ACACGTA550.069.99657438
TTTACAC550.069.9965742
ATACGAG353.7052814E-969.99657412
GACTAAT353.7052814E-969.99657449
TCGCCTA150.002224182769.9965736
AAGTATC252.8381437E-669.9965740
TATCACG252.8381437E-669.9965743
GGAATGT150.002224182769.9965720
CAGAACC150.002224182769.996574
ACACGAT150.002224182769.9965720
CTCGTGT150.002224182769.9965739
GATCGTA150.002224182769.9965738
Produced by FastQC (version 0.11.5)