FastQCFastQC Report
Thu 26 May 2016
SRR1781342_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781342_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318528
Sequences flagged as poor quality0
Sequence length76
%GC96

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25361379.62031595338559No Hit
CTTATACACATCTGACGCTGCCGACGAAGATCTCTGTGTAGATCTCGGTG12420.38991862567811936No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4970.15603023909985936No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4620.145042194092827No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4370.13719359051637534No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3450.10831072935503314No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3330.10454339963833635No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCCT150.002219288170.0328537
CCACAAA150.002219288170.0328542
GCCACAA150.002219288170.0328541
TGGGTCT150.002219288170.0328539
CTCGACT150.00222067270.0218454
ACAAACT150.00222067270.0218444
TCTATAT150.00222067270.0218444
CGGTGTA301.0197073E-770.0218434
GACTGCC150.00222067270.0218435
CTCCGTG150.00222067270.0218447
CAAAATG150.00222067270.0218456
TGGTCCT150.00222205770.0108465
GGTCCTG150.00222205770.0108466
CAGTAAG150.00222205770.0108461
TAAGAAA207.913752E-570.0108464
AGGGATC150.00222205770.0108457
GATGAAA150.00222205770.0108461
ATCAGTA207.913752E-570.0108459
ACCTGGT150.00222205770.0108462
TCAGTGT150.00222205770.0108433
Produced by FastQC (version 0.11.5)