FastQCFastQC Report
Thu 26 May 2016
SRR1781342_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781342_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318528
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT17123453.75791139240506No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG208876.557351316053847No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC82802.599457504520796No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCAGATCTCGTATGCCGT73832.3178496081977094RNA PCR Primer, Index 47 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC39991.2554626280892105No Hit
ATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCTT30380.953762306610408Illumina PCR Primer Index 9 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT26220.823161543098252RNA PCR Primer, Index 47 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22480.7057464335945348No Hit
CTAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT20390.6401321076953989RNA PCR Primer, Index 47 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC17540.5506580269238498RNA PCR Primer, Index 47 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGCTCTCGTATGCCGT15930.5001130198915009No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT15090.4737417118746232No Hit
ATTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT12520.39305806710870006RNA PCR Primer, Index 47 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG12190.3826979103877838No Hit
CTTTACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC10250.3217927466345188No Hit
TCTTATACACATCTCCGAGCCCACGAGACACTCGCAGATCTCGTATGCCG9650.30295609805103474No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGCTCTCGTATGCCG8450.26528280088406675No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC7940.24927164958810527No Hit
CCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG7700.24173699015471167No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG7490.23514416315049227No Hit
TTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC7350.23074894514767932RNA PCR Primer, Index 47 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGCCCCTCGCAGATCTCGTATGCCGT7330.23012105686156317No Hit
TATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCT5660.17769238497086598No Hit
CTTATACACATCTCCGAGCCAACGAGACCCTCGCAGATCTCGTATGCCGT5110.16042545710267228RNA PCR Primer, Index 47 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATATCGTATGCCGT4320.13562386980108498No Hit
TCTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT4250.1334262607996785No Hit
CTTATACACATCTCCGAGACCACGAGACCCTCGCAGATCTCGTATGCCGT4220.1324844283705043RNA PCR Primer, Index 47 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCCACGCAGATCTCGTATGCCGT4100.1287170986538075No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGCTCTCGTATGCC3790.11898483021900742No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAATCTCGTATGCCGTC3680.11553144464536869No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGA3640.11427566807313642No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGACGT3620.1136477797870203No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGAATGCCGT3570.11207805907172995No Hit
CTTATACACATCTCAGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT3510.11019439421338156RNA PCR Primer, Index 47 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCAGT3370.10579917621056861No Hit
TCATATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG3210.10077606992163954No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTG207.90163E-570.03252470
TACAACC150.002219331570.0325264
TGTACAA150.002219331570.0325262
ACGCGGG150.002219331570.0325269
ACAACCT150.002219331570.0325265
CATGTAC150.002219331570.0325260
AACGGGC252.8284612E-670.0325169
CTTCAAA150.002220714570.0215257
TCATGTA150.002220714570.0215259
TTCAAAA252.8311024E-670.02151558
TGAAATA252.8311024E-670.02151559
TAGAAGA150.002222098470.0105231
ACTCGCT252.8337454E-670.0105229
CACACGC650.070.0105228
CACGCAC150.002222098470.0105230
GACACTA401.3460522E-1070.0105226
ACTTTCT150.002222098470.0105232
ACACACG600.070.0105227
CACTCTC252.8337454E-670.0105228
ACTAGCA401.3460522E-1070.0105229
Produced by FastQC (version 0.11.5)