FastQCFastQC Report
Thu 26 May 2016
SRR1781341_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781341_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1240802
Sequences flagged as poor quality0
Sequence length76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42682134.39880013088309No Hit
CTTATACACATCTGACGCTGCCGACGAAGATCTCTGTGTAGATCTCGGTG38790.31262038584721813No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACG352.9832336E-759.9794122
CGTATCA7450.052.63333556
CGCTAGT200.00694941252.5052668
TATCTCG200.006964938752.475644
TTGTGCG2300.051.721382
TCTCGGT9400.051.4047143
TCGGTGG11450.050.7638245
CGCCGTA8400.050.43391853
CCGTATC7950.050.2078855
GATCTCG9000.049.40977541
GTGGTCG10250.049.18359448
ATCTCGG8900.049.17809342
CGGTGGT11150.048.9893246
TCGCCGT8650.048.9762952
CTCGGTG10200.048.74609844
GGTCGCC9550.048.02496750
TCTGGCG4450.047.957211
TGGTCGC9950.047.85363849
GCCGTAT8550.047.50354854
CGCGTCT2800.047.4798747
Produced by FastQC (version 0.11.5)