FastQCFastQC Report
Thu 26 May 2016
SRR1781341_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781341_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1240802
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT30709624.749798920375692No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG377093.039082786778229No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC181351.4615547041349062No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62400.5029005433582473No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC56220.45309404723719016No Hit
CATATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT41240.33236567961689295No Hit
CTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT37590.30294922155186726No Hit
ATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCTT36560.2946481388650244Illumina PCR Primer Index 6 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT25150.20269148502339618No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGCCATATCTCGTATGCCGT23950.19302032072804526No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCAAATCTCGTATGCCGT20040.1615084437323602No Hit
CTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC19610.1580429431931928No Hit
CTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG16520.1331396951326642No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTAAGCCGT14880.11992243726235129No Hit
ATTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT14830.11951947208337835No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCAGT14390.11597337850841632No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATCTCGTATGCCGTCT13720.11057364511017874RNA PCR Primer, Index 41 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAA150.002225393269.9964524
AGGGGGG385550.067.1623770
AAGCCGT10250.058.38728744
TAACTCG4750.058.20757736
AAGGGGG450450.057.38470569
ACTCGTA4750.056.7339738
TCTCGAA6100.056.2266638
CCGTCTT444500.056.0578947
GCCGTCT448500.056.05179246
CTGCTTG442300.056.02022654
TATCTCG460450.056.00324236
TATCGTC252.3917103E-455.99716640
TGCTTGA445100.055.9845855
CGTCTTC448400.055.94495448
TCTGCTT440600.055.90276753
CTTCTGC443300.055.9017551
GTCTTCT443700.055.88290449
ATATCTC461200.055.8666435
TATGCCG455400.055.8480743
TTCTGCT440100.055.83109752