FastQCFastQC Report
Thu 26 May 2016
SRR1781340_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781340_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546481
Sequences flagged as poor quality0
Sequence length76
%GC95

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43704179.97368618488109No Hit
CTTATACACATCTGACGCTGCCGACGAAGATCTCTGTGTAGATCTCGGTG39600.7246363551523292No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11950.2186718293957155No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9490.17365654066655564No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8210.15023395140910661No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7430.13596081108034863No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7010.12827527398024818No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6920.12662837317308379No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5810.10631659655138971No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGCTC150.002219136370.0408540
TTCACTC150.002219136370.0408542
TCACTCT150.002219136370.0408543
CACTCTG150.002219942670.0344444
GATGAAC150.002219942670.0344444
ATCAGGA207.916377E-570.01520559
TTACTGT207.916377E-570.01520553
TTGAGTT207.916377E-570.01520548
GTAGCAT252.835337E-670.01520557
TACTGTC207.916377E-570.01520554
CGTAGCA207.916377E-570.01520556
CGTGTGA207.916377E-570.01520556
GAGTTAC207.916377E-570.01520550
AGTTACT207.916377E-570.01520551
TCAGTAA252.835337E-670.01520560
CCTGGTC150.00222236370.015253
CGGTAAG150.00222236370.015261
GTGCAGT207.9199686E-570.008833
TCTCTGA150.002223977670.0023831
AACCAAG150.002223977670.0023831