FastQCFastQC Report
Thu 26 May 2016
SRR1781340_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781340_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546481
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT32162658.8540132227836No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG403057.3753707814178355No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC164563.0112666314107903No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62791.1489877964650188No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC61761.1301399316719154No Hit
CATATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT44070.806432428574827No Hit
CTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT38610.706520446273521No Hit
ATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCTT34050.6230774720438588RNA PCR Primer, Index 20 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT27010.4942532311278892No Hit
CTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC21570.3947072267837308No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGACATCTCGTATGCCGT19580.3582924200475405No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCAATCTCGTATGCCGT19150.35042389396886625No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG17370.31785185578272623No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAAAGCCATCTCGTATGCCGT16970.3105322966397734No Hit
ATTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT15290.27979014823937154No Hit
CCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG14410.26368711812487533No Hit
CTTATACACATCTCCGAGCCCACGAGACGAACTGCCATCTCGTATGCCGT12720.23276198074589968No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGACGT11000.20128787643120255No Hit
CTTTACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC10930.2000069535811858No Hit
CTTATACACATCTCCGAGCCAACGAGACGAAATGCCATCTCGTATGCCGT10340.1892106038453304No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTAAGCCGT9750.17841425410947498No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC9710.1776822981951797No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGA8660.15846845544492855No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCAGT8370.15316177506628775No Hit
CTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG8050.1473061277519255No Hit
TTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC8030.14694014979477787No Hit
CTTATACACATCTCCGAGACCACGAGACGAAATGCCATCTCGTATGCCGT7630.13962059065182503No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGAATGCCGT7160.13102010865885547No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCATCTCGTATGCCGTC7150.13083711968028164No Hit
TATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCT6410.11729593526581894No Hit
TCATATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG6350.11619800139437601No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCACGTATGCCGT6060.11089132101573522No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATATCGTATGCCGT5870.10741453042283264No Hit
CTTATACACATCTCAGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT5580.10210785004419183No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAAGGG207.91413E-570.0192267
AGGGGCG401.3460522E-1070.0192270
AATGGTG150.002222664270.0128169
AAGGGGA150.002222664270.0128169
TAACTCA207.917718E-570.012811
CCCTGGA150.002222664270.012811
GTACACA207.921309E-570.006414
AACTCAA207.921309E-570.006412
TGTACAC207.921309E-570.006413
CGTGAAA150.002223471270.006458
TACGCAT353.7052814E-970.00645
GAAACCG150.002223471270.006460
GTGAAAC150.002223471270.00642
TACACAG252.8374561E-670.00645
GAGTGTG150.002224278470.030
GGGTGCA150.002224278470.034
TTGGCCG150.002224278470.056
CTACTTG252.8389986E-670.054
TACTTGA301.0232907E-770.055
CCTAGAC252.8389986E-670.014
Produced by FastQC (version 0.11.5)