FastQCFastQC Report
Thu 26 May 2016
SRR1781339_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781339_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129815
Sequences flagged as poor quality0
Sequence length76
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46996841.596898607294115No Hit
CTTATACACATCTGACGCTGCCGACGAAGATCTCTGTGTAGATCTCGGTG42410.37537118908847905No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15040.13311913897407981No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGG150.002229530669.9632643
TTGTGCG2050.052.9107632
TGCGCGT2400.052.472455
CGCCGTA9700.051.25332353
CGTATCA9650.051.1492856
CGCGTCT2600.051.1277
TTATGCG1100.050.8936352
CCGTATC9500.050.8581955
TATGCGC1050.049.9737633
TGCCGGC4950.049.4733619
ATCTCGG10150.048.99837542
CTGCCGG5550.048.53737318
CGCGTGT1300.048.4361047
GGTCGCC11500.048.4023350
TCGCCGT10150.048.29113852
TCTCGGT11400.048.23409343
GTCGCCG11550.048.19706351
GCCGTAT10050.048.074954
GATCTCG10350.048.0515441
TCGGTGG13750.047.8802945
Produced by FastQC (version 0.11.5)