FastQCFastQC Report
Thu 26 May 2016
SRR1781339_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781339_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129815
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT34520930.554471307249415No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG436613.8644379832096405No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC181361.6052185534799943No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC76690.678783694675677No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68720.6082411722273116No Hit
ATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCTT52480.4645008253563636No Hit
CATATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT49530.4383903559432296No Hit
CTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT43770.3874085580382629No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGAATTATCTCGTATGCCGT37070.32810681394741614No Hit
CTTAAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT29550.26154724446037625No Hit
CTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC24900.2203900638600125No Hit
CTTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG21160.18728729924810697No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATAATCTCGTATGCCGT20860.18463199727389No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAAATATCTCGTATGCCGT18200.16108831976916577No Hit
ATTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT15340.13577444094829685No Hit
CCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG14560.1288706558153326No Hit
CTTTACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC13540.11984262910299473No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC11690.10346826692865646No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG420050.067.31222570
TAACTCG6050.061.89863236
TAGGTAC352.976667E-759.9978836
ACTCGAA1800.058.3312738
TCGTAAG5750.057.8240440
ACTCGTA5750.057.21536338
AAGGGGG494700.057.12654569
CCGACTT5300.056.13009347
CGTCTAG509.786163E-1056.0277751
AAGCCGT5750.055.99801644
CGACTTC5750.055.99801648
CCGTCTT499750.055.86495647
GCCGTCT503750.055.8173846
CGTCTTC504600.055.76497348
CTGCTTG498800.055.76085754
TATGCCG509000.055.74360743
TGCCGTC507150.055.7123145
ATGCCGT509050.055.7106344
CTCGTAT516350.055.70927439
TCTCGTA516250.055.68616538
Produced by FastQC (version 0.11.5)