FastQCFastQC Report
Thu 26 May 2016
SRR1781338_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781338_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993226
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29881230.084995761286958No Hit
CTTATACACATCTGACGCTGCCGACGATTCTAAGTGTGTAGATCTCGGTG34770.3500713835521825No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11030.11105226806386462No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGCG1750.061.9658282
TATACCG452.6988346E-854.4555870
CGATTCG200.00697109452.4631418
CGCCGTA6550.051.3237253
GTGGTCG8300.050.62296348
CGTATCA7200.049.60847556
CCGTATC6950.049.3775455
TTATGCG1000.048.9729962
GATCTCG7800.048.9523341
TCGCCGT7050.048.6771552
TCTCGGT8250.048.40524343
TGGTCGC7600.048.374949
TCGGTGG10050.048.09376545
TATGCGC950.047.8562933
ATCTCGG7950.047.5880742
CTCGGTG8750.047.2382244
CGGTGGT9500.046.82027446
GGTCGCC8100.046.25101550
GCCGTAT7400.045.9016454
CGTCTGG2600.045.73489
Produced by FastQC (version 0.11.5)