FastQCFastQC Report
Thu 26 May 2016
SRR1781338_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781338_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993226
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT16590316.703449164641277No Hit
CATATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT426694.296001111529501No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG334533.3681156151772105No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC133721.346319971486852No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54600.5497238292191304No Hit
CTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT48650.4898180273170457No Hit
CAAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT42010.4229651660347192No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC33650.3387949973117901No Hit
ATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCTT19650.19784016930688483RNA PCR Primer, Index 17 (95% over 22bp)
CACTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC18730.1885774234665625No Hit
TCATATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG13680.1377330033647931No Hit
CATAAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT11220.11296522644393119No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGCTCTCGTATGCCGT11190.11266318038392067No Hit
CCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG10920.10994476584382609No Hit
CTTAAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT10840.10913930968379805No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG267000.067.4523670
CATATAC63000.062.4523541
ATATACA68900.059.1336822
TCGTAAG4950.057.26844840
ACTCGTA4450.056.6249838
AAGGGGG318200.056.44492369
CCGTCTA5000.055.2958647
AATGCCG4750.054.52223243
ATGCCGA4650.054.189544
GATCTCG328450.054.03310436
GCCGTCT325500.054.01746746
ATCTCGT325350.054.01009837
TATGCCG328250.054.00205243
ATGCCGT328450.053.99048244
CCGTCTT321150.053.9863147
TGCCGTC327350.053.9473945
CTCGTAT330600.053.8299139
GTATGCC329200.053.82495542
CGTCTTC323700.053.80969248
TCTCGTA331000.053.77543638