FastQCFastQC Report
Thu 26 May 2016
SRR1781337_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781337_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1222201
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39730432.50725535325204No Hit
CTTATACACATCTGACGCTGCCGACGATTCTAAGTGTGTAGATCTCGGTG64920.5311728594560141No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAC150.002231102769.9511820
CGATACG150.002231102769.9511821
TCGTTCG150.002234363769.925424
TTATGCG553.6379788E-1157.221072
CGTATCA10550.054.10453856
CGCCGTA10750.053.7494553
CCGTATC10850.052.61071455
GCCGTAT11200.051.2793154
GTGGTCG12300.051.24880648
TCGCCGT11300.051.13332752
GGTCGCC11600.051.0184550
ATCTCGG11800.050.47118442
TGGTCGC12050.050.277749
TCTCGGT11700.050.00427243
TATGCGC707.2759576E-1249.9467283
GATCTCG11950.049.83765441
TCGGTGG12600.049.76085345
CTCGGTG12150.049.58988644
CGGTGGT12650.049.0103846
GTCGCCG12050.048.82258651
Produced by FastQC (version 0.11.5)