FastQCFastQC Report
Thu 26 May 2016
SRR1781337_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781337_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1222201
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT20558416.820801161183798No Hit
CATATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT496224.060052315453841No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG384263.144000045818977No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC148641.2161665716195618No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91830.7513494097942973No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCCCAGCATCTCGTATGCCGT83050.679511798795779No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC56280.4604807228925521No Hit
CTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT46900.3837339357437934No Hit
CAAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT38730.31688732049801954No Hit
ATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTCTT31660.2590408615276865RNA PCR Primer, Index 43 (95% over 22bp)
CATATACACATCTCCGAGCCCACGAGACAGCCCAGCATCTCGTATGCCGT30180.24693156035709346No Hit
CACTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC18910.15472086833507745No Hit
CTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC16100.13172956003145145No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGCCCAGCATCTCGTATGCCG15640.12796585831626714No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACAAGCATCTCGTATGCCGT14460.11831114522079429No Hit
CCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG12520.10243814233501691No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG335950.067.07345670
CATATAC80450.062.86191
ATATACA85450.061.0626642
CCGCATA352.974666E-760.00427633
TCGTAAG9900.058.67664740
ACTCGTA3650.058.48774738
AAGCCGT10550.056.38828344
TATCTCG252.3912218E-455.9994136
AAATACA17450.055.952432
CTCGTAA10550.055.0637539
CCGTCTA4450.055.04659747
AAGGGGG408950.054.9931969
CGTAAGC11100.054.54004741
ATCTCGT412850.054.2965937
TCTCGTA413700.054.2335738
CCGTCTT380500.054.20606247
GCCGTCT384350.054.1729546
CTCGTAT408500.054.09927739
CGTCTTC377650.054.04989648
CATCTCG418200.054.03936436
Produced by FastQC (version 0.11.5)