FastQCFastQC Report
Thu 26 May 2016
SRR1781336_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781336_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347841
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37712627.980006543798563No Hit
CTTATACACATCTGACGCTGCCGACGATTCTAAGTGTGTAGATCTCGGTG43490.3226641718125506No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA7600.050.67342856
CCGTATC7650.049.8864355
TTGTGCG1750.047.9790882
CGCCGTA8350.047.8026353
CGGTGGT9550.046.19560246
ATCTCGG8350.046.13391542
TCTCGGT8650.045.7484643
TCGGTGG9950.045.0456245
GTGGTCG9700.044.3983548
CTCGGTG9350.044.19451544
GATCTCG8750.044.02493741
TCGCCGT9000.043.57214752
TGGTCGC9600.043.4019449
GGTCGCC9600.043.03721650
GTCGCCG9800.042.5146151
GCCGTAT9150.041.7098754
CGTCTGG2350.040.192269
GTAGATC9800.039.66532538
AGATCTC10100.039.18060740
TAGATCT10400.038.05039639
Produced by FastQC (version 0.11.5)