FastQCFastQC Report
Thu 26 May 2016
SRR1781336_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781336_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347841
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT20555815.250908675429816No Hit
CATATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT529843.9310274728250585No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG392972.9155516117999083No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC167381.241837872568055No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71150.5278812560235221No Hit
CTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT54220.4022729683990916No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC49160.36473144829397536No Hit
CAAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT46600.3457381100589758No Hit
ATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCTT32600.24186829158632214RNA PCR Primer, Index 24 (95% over 21bp)
CACTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC23470.17413033139665582No Hit
CTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC15710.11655677487181351No Hit
TCATATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG14800.10980523667109103No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG337850.067.03940670
CATATAC80250.061.940821
TTTGACG308.369689E-658.34647864
ATATACA88500.058.01282
AAGGGGG395950.057.0895169
CCGTCTT398550.054.52926647
GCCGTCT402950.054.48967446
TATGCCG406650.054.4327943
TGCCGTC406650.054.4069745
CTGCTTG400800.054.3561454
TGCTTGA403050.054.3499555
GTATGCC407700.054.33552642
TCGTAAG6700.054.32245640
TCTCGTA415900.054.3076438
CTCGTAT413900.054.29948439
ATGCCGT407750.054.29453344
ATCTCGT415800.054.2870337
TAACTCG4450.054.26779636
TATCTCG411650.054.26024636
TCTCGAA6450.054.2576738
Produced by FastQC (version 0.11.5)