FastQCFastQC Report
Thu 26 May 2016
SRR1781335_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781335_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677086
Sequences flagged as poor quality0
Sequence length76
%GC78

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36467253.85903710902308No Hit
CTTATACACATCTGACGCTGCCGACGATTCTAAGTGTGTAGATCTCGGTG45840.6770188720487501No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10930.1614270565334389No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7970.11771030563325782No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC207.916905E-570.01671
GTTTCGC150.002223707370.00635549
GACCGAT150.002226967669.980518
TACGGAG150.002226967669.98057
TCAACGT150.002226967669.980514
TATACCG150.0022276269.975332
TTCGGGA308.364546E-658.3472536
CGCGTCT1000.055.98447
CGTATCA9250.054.4994956
GTGGTCG10150.054.14684748
TGCCGGC3050.053.919419
TCGCCGT9500.053.80230752
GCCGTAT9200.053.27357554
CCGTATC9150.053.18208755
GGTCGCC10250.052.59013750
CGGTGGT11200.052.51252746
GATCTCG9600.052.51252741
TGAATCG200.00694425452.51252757
AATCGGT200.00694628452.50864461
ACGAAGG200.006958472552.4853724