FastQCFastQC Report
Thu 26 May 2016
SRR1781335_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781335_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677086
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT19614828.969436674218635No Hit
CATATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT495347.315761956383679No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG385415.692186812310401No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC152462.251708054811353No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG73381.0837618854916509No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC52890.7811415388887083No Hit
CTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT49230.7270863671675385No Hit
CAAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT40700.6011053248774897No Hit
ATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCTT32360.4779304253817093RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCTTATTATCTCGTATGCCGTC20760.306608023205324No Hit
CACTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC19930.2943496099461516No Hit
CTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC16330.24118058858106653No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG13490.19923613839305496No Hit
TCATATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG12230.18062698091527515No Hit
CTTAAACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT11220.1657101165878485No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATAATCTCGTATGCCGTC11040.16305166551959427RNA PCR Primer, Index 12 (95% over 21bp)
CATAAACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT10780.15921168064322702No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG10650.15729168820504338No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTAATAATCTCGTATGCCGT9870.14577173357594161No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAATTAATCTCGTATGCCGT8470.12509489193396406No Hit
CTTTACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC7480.11047341105856567No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC7370.10884880207241031No Hit
CTTATACACATCTCCGAGCCCACGAGACTCATATTAATCTCGTATGCCGT6940.1024980578538029No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT150.002222739970.0140466
GACGCGT150.002224694269.9985311
GCGGTTG207.9270954E-569.9985232
CTATCGA150.00222534669.99335537
AGGGGGG318050.066.17267670
ATCGAAT800.065.6187739
CATATAC71350.065.58861
ATATACA75000.064.4405442
CGACTAC1200.064.16057648
CAAATAC13850.059.6421361
TCCGACC308.377434E-658.33210413
AAATACA15200.057.0998422
ATATCCG2800.056.25296810
TCAATCG252.3914546E-455.99468223
CCGACTA1250.055.99468247
TCGTAAG4200.055.8280340
CACGTAT2700.055.73544739
AATGCCG3900.055.63574643
TCTTTAT8750.055.6029321
AAGGGGG379000.055.47550269
Produced by FastQC (version 0.11.5)