FastQCFastQC Report
Thu 26 May 2016
SRR1781334_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781334_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477958
Sequences flagged as poor quality0
Sequence length76
%GC90

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34843672.9009661936823No Hit
CTTATACACATCTGACGCTGCCGACGATTCTAAGTGTGTAGATCTCGGTG38100.7971411713999975No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7440.15566221299779479No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6890.14415492574661373No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5730.11988501081685002No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5100.10670393632913353No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5000.10461170228346424No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTTA150.002218108270.04767649
GTCGACA150.002218108270.04767648
TATGGTC150.002218108270.04767638
CGATTTT150.002218108270.04767644
ATGTCGG207.8972E-570.04767642
CACAGTT150.002218108270.04767648
GTTCAAC150.002218108270.04767640
TTACCGT150.002218108270.04767649
ACCGTCC150.002219030270.0403351
CCACAGT150.002219030270.0403347
GCGTATC207.909504E-570.02566555
GTCTATA150.002222720870.0109941
GTTGTGC301.02189006E-770.0109941
CTTATGG150.002222720870.0109941
AGACCAT150.00222456869.9963432
TTGCCTC150.00222549269.98900630
TTATGCG207.934158E-569.981682
TCTATAC207.934158E-569.981682
CAGCGTA150.002230116269.95238515
AGCGTAC150.002230116269.95238516
Produced by FastQC (version 0.11.5)