FastQCFastQC Report
Thu 26 May 2016
SRR1781334_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781334_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477958
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT19390840.570091932763965No Hit
CATATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT4907610.267847802526582No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG362297.579954724055252No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC141522.960929621431172No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61701.2909084061779488No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC54571.1417321187217286No Hit
CTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT47060.9846053418919655No Hit
CAAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT37140.7770557245615723No Hit
ATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCTT27110.5672046497809431Illumina PCR Primer Index 10 (95% over 21bp)
CACTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC20140.421375936797794No Hit
CTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC15760.3297360855974793No Hit
CCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG12050.2521142025031488No Hit
CTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG11710.24500060674787325No Hit
TCATATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG11220.23474865992409374No Hit
CTTATACACATCTCCGAGCCCACGAGACTGAGCCTTATCTCGTATGCCGT11060.23140108545102292No Hit
CTTAAACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT9890.20692194711669226No Hit
CATAAACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT9090.190184074751338No Hit
CTTTACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC8090.16926173429464514No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC7740.16193891513480266No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGACTTATCTCGTATGCCGT6870.14373647893747987No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTAATCTCGTATGCCGT6690.13997045765527516No Hit
ACTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG6330.1324384150908657No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCATTATCTCGTATGCCGT6210.1299277342360626No Hit
TTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC5740.12009423422141695No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG5580.1167466597483461No Hit
CATATACACATCTCCGAGCCCACGAGACTGAGCCTTATCTCGTATGCCGT5480.11465442570267681No Hit
TATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCT5240.10963306399307052No Hit
ATTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT5190.10858694697023588No Hit
AATATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT4850.10147335121496032No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTCG150.00222103770.0243870
AGGGCGG207.910226E-570.0243870
ACAGTGA252.8378745E-670.002427
GCAGGGA207.922536E-570.002460
GACTCAG150.002223804170.002426
AATGTGA353.7071004E-970.002427
ATTCCGT252.839639E-669.9950944
ATATCAG401.3460522E-1069.9950910
CCATAAC500.069.9950933
ACGACTA252.839639E-669.9950947
TATCCCG252.839639E-669.9950943
GTGCCAA401.3460522E-1069.9950930
ACACGAT150.00222472769.9950820
GTCGATT150.00222472769.9950840
CTCCGAC150.00222472769.9950812
TTTCGGT150.00222472769.9950845
ATCAGAG600.069.9950812
ACTCTCG150.00222472769.9950836
ACTCTAA150.00222472769.9950838
CTCCCAG150.00222472769.9950812
Produced by FastQC (version 0.11.5)